Sanger Sequencing and Fragment Analysis Service

 

***** ATTN: Sanger Sequencing Change in Services – Effective Monday, February 12, 2018 *****

Please visit the Sanger Sequencing – Eurofins page for important information regarding this change.


The Center for Genome Innovation is pleased to announce the addition of Sanger Sequencing and STR Analysis (new service to support NIH sample Authentication requirements) as part of our service portfolio effective Monday, November 28th, 2016.

Samples for Sanger Sequencing and STR Fragment analysis can be submitted via dropbox to the CGI (sample dropbox location outside of third floor elevators, Engineering/Science Building [NESB]). The new building is between the Chemistry and Pharmacy buildings on 181 Auditorium Road. All submissions must be accompanied by completed online form submission found here

See table below for a complete list of service types as well as descriptions for each service. NOTE: The per sample rates listed in the table below are internal rates. Please contact the CGI for external pricing for non-UCONN members.

Sanger Sequencing DropBox Submission Policies

Samples for run-only Sanger sequencing should be dropped off directly to the CGI by 1:00pm for processing that day.  Samples must be placed inside a freezer box or in a zip top plastic bag CLEARLY labeled with YOUR NAME, PI name and submission date.

  • Be sure to complete the online submission form before dropping off your samples!
  • All samples being dropped of must comply with CGI submission guidelines (see below)

Commonly Used Sequencing Primers

The CGI can provide aliquots of commonly used sequencing primers at the customer’s request. Here are the primers and their sequences currently available:

Short Tandem Repeat (STR) Fragment Analysis

Effective January 2016, the National Institutes of Health (NIH) Office of Extramural Research (OER) revised grant application requirements as part of the program to “Enhance Rigor and Reproducibility.” Included in this requirement are four components the NIH has considered important for enhancing rigor and transparency. Among these is the “Authentication of Key Biological and/or Chemical Resources”, which must be outlined in all new grant applications and implemented in grants funded from 2016 onward. To summarize the full description1, the NIH requires that resources used to conduct NIH funded research must be authenticated on a regular basis. These resources include all cell lines and other biologics, including but not limited to primary cell lines, established cell lines and those obtained from another source regardless of the source (company with their own sample authentication2, non-NIH funded material source, etc). Specifically, “If key resources have been purchased or obtained from an outside source that provided data on prior authentication, the investigator is still expected to provide their own authentication plans for these key resources.” Moreover, the authentication requirement applies to cell lines and biologics that may differ from laboratory to laboratory or may differ over time (e.g. in culture).

The NIH has not set a standard for this authentication; rather, the scientific community is empowered to provide this standard. However, the NIH has provided a recommendation that cell lines may be authenticated by chromosomal analysis and/or short tandem repeat (STR) profiling.

The CGI and Chromosome Core (Institute for Systems Genomics) provide several services to meet this new NIH requirement. Our newest and most affordable service is forensic-standard STR typing of samples. Within our portfolio, we also offer more detailed assessment of samples, including manual karyotyping and Affymetrix SNP Cytoscan Arrays.

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1 The quality of the resources used to conduct research is critical to the ability to reproduce the results. NIH expects that key biological and/or chemical resources will be regularly authenticated to ensure their identity and validity for use in the proposed studies.  Key biological and/or chemical resources may or may not be generated with NIH funds and: 1) may differ from laboratory to laboratory or over time; 2) may have qualities and/or qualifications that could influence the research data; and 3) are integral to the proposed research. These include, but are not limited to, cell lines, specialty chemicals, antibodies and other biologics. Researchers should transparently report on what they have done to authenticate key resources, so that consensus can emerge.

2  http://grants.nih.gov/reproducibility/faqs.htm#4850: “If key resources have been purchased or obtained from an outside source that provided data on prior authentication, the investigator is still expected to provide their own authentication plans for these key resources.”