All instrumentation is accessible to faculty and students pending appropriate training. The extent of training needed before independent use depends on the instrumentation and skill level of the user. Training is required for all next generation sequencing platforms (if users would like to use instruments independently) and can be met by either attending a workshop or setting up one-on-one training with CGI staff. If individuals are not comfortable working independently, he/she can request supervision from the CGI staff.
In addition to instrument access, the CGI also offers fee-for-service for NextGen sequencing and genotyping services. A full list of services and associated costs can be found by visiting the CGI Service Rates page. If you have questions or would like more information about any of the services provided by the CGI, contact Bo Reese.
Sequencing and Genome Analysis:
• Illumina NovaSeq 6000 (x1)
• Illumina MiSeq (x2)
• Illumina NextSeq 550 (x1)
• Bionano Irys (for optical mapping)
• Oxford NanoPore MinION and PromethION
Solution-Phase, Real-Time and Digital PCR:
• ABI GeneAmp 9700 gold block thermocyclers
• BioRad C1000 Thermal Cyclers (deep well reaction modules)
• BioRad CFX96 Real-Time PCR machine (x2)
• QX200 Digital Droplet PCR Droplet Generator and Reader (BioRad)
Sample QC:
• Agilent 2100 Bioanalyzer Automated Electrophoresis system
• Agilent 2200 and 4200 TapeStation Automated Electrophoresis system
• Thermo-Fisher NanoDrop 2000
• Agarose gel electrophoresis systems
• Turner Biosystems Picofluorometer
• Qubit 3.0 (various assays)
Nucleic Acid Manipulation:
• Digilab Hydroshear
• MP Biomedicals FastPrep 24 Sample Prep System
• Qiagen Tissuelyser II
• Covaris S2
• Sage Science Pippin Prep, PippinHT and Blue Pippin
Array Systems:
• Illumina NextSeq 550 (w/ Infinium Arrays for Human CytoSNP 850K and Methylation EPIC)
Single Cell Genomics:
• 10X Chromium System (10X Genomics; Single Cell Analysis and other applications)
Other equipment:
• IKA UltraTurrax
• Allegra X-12R refrigerated centrifuge
• Benchmark Plate Shaker
• BioRad Gel Doc gel imaging system