Center for Genome Innovation – FEE STRUCTURE Fiscal Year 2016 – 2017
- These rates are based on an internal audit of costs and are subject to annual review. Rates for internal UCONN researchers are shown. Rates billed to external (non-UCONN) customers must include institutional indirect costs even though these costs are not included in the rates for internal University users. The FY16-17 rate adds an additional 58% to billable services.
- Projects are not limited to the sample numbers shown in each section. For sample pricing greater than numbers shown, please contact the CGI.
MAIN SERVICES:
Illumina Library Preparation and Sequencing (includes applications for the NextSeq 500, MiSeq and HiSeq Sequencing platforms) (CGI, UCONN Storrs on-site and UCONN Health pricing)
A. Library Preparation workflows (Assisted Rates):
1. TruSeq/EpiGnome Methyl-Seq Library Sample Prep including Bisulfite conversion ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| $493.86 | $590.44 | $687.01 | $783.59 | $880.17 | $976.74 | $1,073.32 | $1,169.90 | $1,267.45 | $1,364.03 | $1,460.60 | $1,557.18 |
2. NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible) ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| $693.58 | $781.82 | $870.05 | $958.29 | $1,046.53 | $1,134.77 | $1,223.00 | $1,311.24 | $1,401.43 | $1,489.66 | $1,557.90 | $1,666.14 |
3. TruSeq Stranded Total RNA Library Prep ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| $745.74 | $881.47 | $1,017.19 | $1,152.92 | $1,288.65 | $1,424.37 | $1,560.10 | $1,695.82 | $1,883.50 | $1,969.23 | $2,104.95 | $2,240.68 |
4. TruSeq DNA Nano Library Prep (low input)——— Number of Samples (top row); Total cost for # of samples shown (bottom row)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| $452.53 | $498.79 | $545.04 | $591.30 | $637.56 | $683.82 | $730.07 | $776.33 | $823.56 | $869.82 | $916.08 | $962.34 |
5. TruSeq Stranded mRNA Library Sample Prep ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| $665.49 | $735.48 | $805.48 | $875.47 | $945.46 | $1,015.46 | $1,085.45 | $1,155.45 | $1,227.39 | $1,297.38 | $1,367.38 | $1,437.37 |
6. Reduced Representation Bisulfite Sequencing using TruSeq DNA Nano Library Sample Prep ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| $746.47 | $808.25 | $870.02 | $931.79 | $993.57 | $1,055.34 | $1,117.12 | $1,178.89 | $1,241.64 | $1,303.41 | $1,365.19 | $1,426.96 |
7. TruSeq Exome Rapid Capture Library Sample Prep (Human Only) ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| $641.57 | $754.47 | $868.07 | $981.67 | $1,095.26 | $1,208.86 | $1,322.46 | $1,436.06 | $1,551.60 | $1,665.20 | $1,778.80 | $1,892.39 |
8. TruSeq ChIP Library Sample Prep ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| $515.63 | $604.69 | $693.75 | $782.81 | $871.86 | $960.92 | $1,049.98 | $1,139.04 | $1,230.04 | $1,319.10 | $1,408.16 | $1,497.22 |
9. Illumina Amplicon Library Sample Prep (using custom amplicon primers) ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| $288.12 | $314.97 | $341.81 | $368.65 | $395.49 | $422.34 | $449.18 | $476.02 | $504.81 | $531.65 | $558.50 | $585.34 |
B. Sequencing Instrument Access Fees for the Illumina NextSeq 500:
1. Unassisted Sequencing Instrument Access:
Single Sequencing Run Access Fee: $294.00 (Flat Rate)
2. Assisted Sequencing Instrument Access:
Single Sequencing Run Access Fee w/ Labor: $324.00 (Flat Rate)
C. Sequencing Reagent Kit Costs for the Illumina NextSeq 500:
Additional Costs for Full Service Component – choose one kit as applicable for run type: (reagent cost per contract price to UConn at time of service):
Illumina NextSeq 500 – 300 cycle (High Output) – $4,094.00
Illumina NextSeq 500 – 300 cycle (Mid Output) – $1,564.00
Illumina NextSeq 500 – 150 cycle (High Output) – $2,557.60
Illumina NextSeq 500 – 150 cycle (Mid Output) – $975.20
Illumina NextSeq 500 – 75 cycle (High Output) – $1,334.00
D. Sequencing Instrument Access Fees for the Illumina MiSeq:
1. Unassisted Sequencing Instrument Access:
Single Sequencing Run Access Fee: $121.00 (Flat Rate)
2. Assisted Sequencing Instrument Access:
Single Sequencing Run Access Fee w/ Labor: $161.00 (Flat Rate)
E. Sequencing Reagent Kit Costs for the Illumina MiSeq:
Additional Costs for Full Service Component – choose one kit as applicable for run type: (reagent cost per contract price to UConn at time of service):
MiSeq Reagent Kit v2 (50 cycle) – $754.40
MiSeq Reagent Kit v2 (300 cycle) – $961.40
MiSeq Reagent Kit v2 (500 cycle) – $1,076.40
MiSeq Reagent Kit v3 (150 cycle) – $805.00
MiSeq Reagent Kit v3 (600 cycle) – $1,407.60
MiSeq Reagent Kit Nano v2 (300 cycle) – $266.80
MiSeq Reagent Kit Nano v2 (500 cycle) – $322.00
MiSeq Reagent Kit Micro v2 (300 cycle) = $404.80
BioRad CFX 96 Real-Time PCR System (CGI, UCONN Storrs on-site and UCONN Health pricing)
A. Instrument Access Fee for the BioRad CFX 96:
1. Unassisted/Assisted Instrument Access:
Single Run Access Fee: $12.00 (Flat Rate per use)
B. Additional Costs (if applicable):
1. CGI Supplied Consumables:
BioRad iQ 96-well Real-time PCR Plate: $5.97 (cost for one plate)
BioRad Optical Sealing Tape: $0.96 (cost for one seal)
Sanger Sequencing and Fragment Analysis (CGI, UCONN Storrs on-site and UCONN Health pricing)
B. Additional Costs (if applicable):
1. CGI Supplied Consumables:
96-well ABI Sequencing Plate: $2.20 (cost for one plate)
Training (CGI, UCONN Storrs on-site and UCONN Health pricing)
A. Workshop Fees:
Flat Rate for Laboratory Workshops (various topics): $850.00
Flat Rate for Bioinformatics Workshops: $500.00
Fluidigm BioMark HD (CGI, UCONN Storrs on-site and UCONN Health pricing)
A. Instrument Access Fee for the Fluidigm BioMark HD:
1. Unassisted/Assisted Instrument Access:
Flat Rate User Access Fee (per array): $155.00
Fluidigm C1 (CGI, UCONN Storrs on-site and UCONN Health pricing)
A. Instrument Access Fee for the Fluidigm C1:
1. Unassisted/Assisted Instrument Access:
Flat Rate User Access Fee (per array): $191.00
B. Single Cell mRNA-Seq:
1. Single Cell Capture and mRNA Library Preparation for Illumina Sequencing:
Please contact the CGI for a price quote
Agilent TapeStation 2200 (CGI, UCONN Storrs on-site and UCONN Health pricing)
A. Instrument Access Fee for the Agilent TapeStation 2200:
1. Unassisted/Assisted Instrument Access:
Flat Rate User Access Fee (per run): $12.00
B. Assay Types (Agilent TapeStation 2200):
1. Genomic DNA
2. High Sensitivity RNA
3. DNA High Sensitivity 1000
4. DNA 5000
Each assay is designed to process a minimum of 2 samples, up to 96. Please contact the CGI for a price based on assay type as well as number of samples.