Center for Genome Innovation – Service Rates (updated Feb 2022)
- Service rates are based on an internal audit of costs for reagents, consumables and labor and are subject to periodic review. Internal rates for UCONN researchers are shown. Rates billed to external (non-UCONN, not for-profit) adds an additional 35% to all billable services for external users. UCONN adds an additional 100% to all billable services for external for-profit company customers.
- Please contact the CGI for project quotes. Projects can be submitted at any time for any number of samples. Project deliverables will be communicated at the time of sample delivery and are subject to change.
MAIN SERVICES:
Illumina Library Preparation and Sequencing (full-service applications for all Illumina Sequencing platforms). NOTE: Please inquire about costs for your specific project and sample number. Select sample number categories are provided to give an idea of costs.
Library Preparation workflows (Assisted Rates):
1. Illumina Stranded Total RNA library preparation, includes ribosomal depletion. ——— Number of Samples (top row); Cost per sample (bottom row)
| 1-6 samples | 12 | 24 | 48 |
| $270.28/sample | $206.82/sample | $173.17/sample | $157.31/sample |
2. Illumina Stranded mRNA library preparation, includes polyA selection. ——— Number of Samples (top row); Cost per sample (bottom row)
| 1-6 samples | 12 | 24 | 48 |
| $187.71/sample | $128.87/sample | $98.16/sample | $83.45/sample |
3. Zymo-Seq RiboFree Total RNA library preparation, includes enzymatic ribosomal depletion for prokaryotic or mixed samples. ——— Number of Samples (top row); Cost per sample (bottom row)
| 1-6 samples | 12 | 24 | 48 |
| $203.01/sample | $158.31/sample | $134.03/sample | $122.85/sample |
4. TruSeq DNA Nano Library Prep (Covaris Shearing) ——— Number of Samples (top row); Cost per sample (bottom row)
| 1-6 samples | 12 | 24 | 48 |
| $129.55/sample | $87.25/sample | $65.43/sample | $54.85/sample |
5. NexteraXT or NexteraFlex (enzymatic shearing) ——— Number of Samples (top row); Cost per sample (bottom row)
| 1-6 samples | 12 | 24 | 48 |
| $88.42/sample | $73.06/sample | $64.71/sample | $60.87/sample |
6. Double-Digest Reduced Representation Bisulfite Sequencing (dRRBS) ——— Number of Samples (top row); Cost per sample (bottom row)
| 1-6 samples | 12 | 24 | 48 |
| $201.86/sample | $130.50/sample | $94.16/sample | $76.32/sample |
7. NexteraFlex for Enrichment (Exome Sequencing) ——— Number of Samples (top row); Cost per sample (bottom row)
| 1-6 samples | 12 | 24 | 48 |
| $239.99/sample | $169.24/sample | $141.10/sample | $127.70/sample |
8. NEBNext Small RNA Library preparation ——— Number of Samples (top row); Cost per sample (bottom row)
| 1-6 samples | 12 | 24 | 48 |
| $206.56/sample | $147.76/sample | $117.06/sample | $102.36/sample |
9. Targeted amplicon/metabarcoding (using custom amplicon primers) ——— Number of Samples (top row); Cost per sample (bottom row)
| 1-24 samples | 48 | 96 |
| $35.57/sample | $31.23/sample | $29.19/sample |
The Center for Genome Innovation also offers NGS sample preparation solutions for the following applications:
- ChIP-Seq (“standard input” and “low input” using Swift Biosciences kits)
- Whole Genome Methylation
- Agilent SureSelect for custom enrichment
- Low input RNA-Seq with various Takkara Bio kits
- Genotyping by Sequencing (ddRAD)
- Single Cell RNA-Seq (10x Chromium)
For more information on pricing for these applications or to inquire about assistance in developing an application that is not shown please contact us!
Sequencing Instrument Access Fees for the Illumina NovaSeq 6000:
Sequencing Instrument Access:
Single Sequencing Run Access Fee w/ Labor: $490.00
Sequencing Reagent Kit Costs for the Illumina NovaSeq 6000 (Prices shown are for full runs. Lane sharing is available. Please inquire for single lane costs and other important project considerations):
Reagent costs change yearly and are also subject to a 4% charge for shipping. Rates are internal per contract price to UCONN at time of service. Prices below are current through Feb 2023:
NovaSeq SP v1.5 100 cycle = $2,153
NovaSeq SP v1.5 200 cycle = $2,819
NovaSeq SP v1.5 300 cycle = $3,075
NovaSeq SP v1.5 500 cycle = $4,305
NovaSeq S1 v1.5 100 cycle = $3,946
NovaSeq S1 v1.5 200 cycle = $4,971
NovaSeq S1 v1.5 300 cycle = $5,381
NovaSeq S2 v1.5 100 cycle = $7,431
NovaSeq S2 v1.5 200 cycle = $9,225
NovaSeq S2 v1.5 300 cycle = $9,840
NovaSeq S4 v1.5 35 cycle = $10,763
NovaSeq S4 v1.5 200 cycle = $13,248
NovaSeq S4 v1.5 300 cycle = $14,760
Lane Sharing is available on all NovaSeq kits. SP, S1 and S2 flow cells have 2 lanes; S4 flow cells have 4 lanes. An Xp lane loading kit is necessary to separate pools:
Xp 2-lane loading kit = $306.00
Xp 4-lane loading kit = $618.00
Sequencing Instrument Access Fees for the Illumina NextSeq 550:
1. Unassisted Sequencing Instrument Access:
Single Sequencing Run Access Fee: $263.50
2. Assisted Sequencing Instrument Access:
Single Sequencing Run Access Fee w/ Labor: $301.00
Sequencing Reagent Kit Costs for the Illumina NextSeq 550:
Reagent costs change yearly and are also subject to a 4% charge for shipping. Rates are internal per contract price to UCONN at time of service. Prices below are current through Feb 2023:
NextSeq 550 v2.5 – 300 cycle (High Output) = $4,776.64
NextSeq 550 v2.5 – 300 cycle (Mid Output) = $1,829.88
NextSeq 550 v2.5 – 150 cycle (High Output) = $2,979.88
NextSeq 550 v2.5 – 150 cycle (Mid Output) = $1,136.20
NextSeq 550 v2.5 – 75 cycle (High Output) = $1,555.72
Sequencing Instrument Access Fees for the Illumina MiSeq:
1. Unassisted Sequencing Instrument Access:
Single Sequencing Run Access Fee: $118.50 (v2/v3 runs); $78.00 (Nano or Micro runs)
2. Assisted Sequencing Instrument Access:
Single Sequencing Run Access Fee w/ Labor: $156.00 (v2/v3 runs); $78.00 (Nano or Micro runs)
Sequencing Reagent Kit Costs for the Illumina MiSeq:
Reagent costs change yearly and are also subject to a 4% charge for shipping. Rates are internal per contract price to UCONN at time of service. Prices below are current through Feb 2023:
MiSeq Reagent Kit v2 (50 cycle) = $858.36
MiSeq Reagent Kit v2 (300 cycle) = $1,098.48
MiSeq Reagent Kit v2 (500 cycle) = $1,235.56
MiSeq Reagent Kit v3 (150 cycle) = $952.20
MiSeq Reagent Kit v3 (600 cycle) = $1,608.16
MiSeq Reagent Kit Nano v2 (300 cycle) = $306.36
MiSeq Reagent Kit Nano v2 (500 cycle) = $368.00
MiSeq Reagent Kit Micro v2 (300 cycle) = $461.84
Instrument Access Fee for the BioRad CFX 96:
1. Unassisted/Assisted Instrument Access:
Access Fee: $15.00/day
Instrument Access Fee for the 10X Chromium:
1. Unassisted/Assisted Instrument Access:
Access Fee: $100
Please inquire about sample preparation costs for single cell RNA-Seq.
Instrument Access Fee for the BioRad Digital Droplet PCR QX200:
1. Unassisted/Assisted Instrument Access:
Access Fee: $100
Cost for sample processing (EvaGreen or probe-based assays) = $5.00/sample
Sample Extraction Services
Qiagen RNA Extraction (homogenized tissues or cells) = $25.00/sample
Qiagen RNA Extraction (PAXGene blood) = $33.00/sample
Qiagen DNA Extraction = $19.00/sample