Center for Genome Innovation – FEE STRUCTURE Fiscal Year 2019
- These rates are based on an internal audit of costs and are subject to perodic review. Rates for internal UCONN researchers are shown. Rates billed to external (non-UCONN) customers must include institutional indirect costs even though these costs are not included in the rates for internal University users. The FY19 rate adds an additional 35% to all billable services.
- Projects are not limited to the sample numbers shown in each section. For sample pricing greater than numbers shown, please contact the CGI.
MAIN SERVICES:
Illumina Library Preparation and Sequencing (includes applications for the NextSeq 500, MiSeq and HiSeq Sequencing platforms) (CGI, UCONN Storrs on-site and UCONN Health pricing)
A. Library Preparation workflows (Assisted Rates):
1. TruSeq/EpiGnome Methyl-Seq Library Sample Prep including Bisulfite conversion ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| $493.86 | $590.44 | $687.01 | $783.59 | $880.17 | $976.74 | $1,073.32 | $1,169.90 | $1,267.45 | $1,364.03 | $1,460.60 | $1,557.18 |
2. NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible) ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| $693.58 | $781.82 | $870.05 | $958.29 | $1,046.53 | $1,134.77 | $1,223.00 | $1,311.24 | $1,401.43 | $1,489.66 | $1,557.90 | $1,666.14 |
3. TruSeq Stranded Total RNA Library Prep ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| $745.74 | $881.47 | $1,017.19 | $1,152.92 | $1,288.65 | $1,424.37 | $1,560.10 | $1,695.82 | $1,883.50 | $1,969.23 | $2,104.95 | $2,240.68 |
4. TruSeq DNA Nano Library Prep (low input)——— Number of Samples (top row); Total cost for # of samples shown (bottom row)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| $452.53 | $498.79 | $545.04 | $591.30 | $637.56 | $683.82 | $730.07 | $776.33 | $823.56 | $869.82 | $916.08 | $962.34 |
5. TruSeq Stranded mRNA Library Sample Prep ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| $665.49 | $735.48 | $805.48 | $875.47 | $945.46 | $1,015.46 | $1,085.45 | $1,155.45 | $1,227.39 | $1,297.38 | $1,367.38 | $1,437.37 |
6. Reduced Representation Bisulfite Sequencing using TruSeq DNA Nano Library Sample Prep ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| $746.47 | $808.25 | $870.02 | $931.79 | $993.57 | $1,055.34 | $1,117.12 | $1,178.89 | $1,241.64 | $1,303.41 | $1,365.19 | $1,426.96 |
7. TruSeq Exome Rapid Capture Library Sample Prep (Human Only) ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| $641.57 | $754.47 | $868.07 | $981.67 | $1,095.26 | $1,208.86 | $1,322.46 | $1,436.06 | $1,551.60 | $1,665.20 | $1,778.80 | $1,892.39 |
8. TruSeq ChIP Library Sample Prep ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| $515.63 | $604.69 | $693.75 | $782.81 | $871.86 | $960.92 | $1,049.98 | $1,139.04 | $1,230.04 | $1,319.10 | $1,408.16 | $1,497.22 |
9. Illumina Amplicon Library Sample Prep (using custom amplicon primers) ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| $288.12 | $314.97 | $341.81 | $368.65 | $395.49 | $422.34 | $449.18 | $476.02 | $504.81 | $531.65 | $558.50 | $585.34 |
B. Sequencing Instrument Access Fees for the Illumina NextSeq 500:
1. Unassisted Sequencing Instrument Access:
Single Sequencing Run Access Fee: $294.00 (Flat Rate)
2. Assisted Sequencing Instrument Access:
Single Sequencing Run Access Fee w/ Labor: $324.00 (Flat Rate)
C. Sequencing Reagent Kit Costs for the Illumina NextSeq 500:
Additional Costs for Full Service Component – choose kit(s) as applicable for run type: (reagent costs change yearly, per contract price to UConn at time of service):
Illumina NextSeq 500 – 300 cycle (High Output)
Illumina NextSeq 500 – 300 cycle (Mid Output)
Illumina NextSeq 500 – 150 cycle (High Output)
Illumina NextSeq 500 – 150 cycle (Mid Output)
Illumina NextSeq 500 – 75 cycle (High Output)
D. Sequencing Instrument Access Fees for the Illumina MiSeq:
1. Unassisted Sequencing Instrument Access:
Single Sequencing Run Access Fee: $121.00 (Flat Rate)
2. Assisted Sequencing Instrument Access:
Single Sequencing Run Access Fee w/ Labor: $161.00 (Flat Rate)
E. Sequencing Reagent Kit Costs for the Illumina MiSeq:
Additional Costs for Full Service Component – choose kit(s) as applicable for run type: (reagent costs change yearly, per contract price to UConn at time of service):
MiSeq Reagent Kit v2 (50 cycle)
MiSeq Reagent Kit v2 (300 cycle)
MiSeq Reagent Kit v2 (500 cycle)
MiSeq Reagent Kit v3 (150 cycle)
MiSeq Reagent Kit v3 (600 cycle)
MiSeq Reagent Kit Nano v2 (300 cycle
MiSeq Reagent Kit Nano v2 (500 cycle)
MiSeq Reagent Kit Micro v2 (300 cycle)
BioRad CFX 96 Real-Time PCR System (CGI, UCONN Storrs on-site and UCONN Health pricing)
A. Instrument Access Fee for the BioRad CFX 96:
1. Unassisted/Assisted Instrument Access:
Single Run Access Fee: $12.00 (Flat Rate per use)
B. Additional Costs (if applicable):
1. CGI Supplied Consumables:
BioRad iQ 96-well Real-time PCR Plate: $5.97 (cost for one plate)
BioRad Optical Sealing Tape: $0.96 (cost for one seal)
Sanger Sequencing and Fragment Analysis (CGI, UCONN Storrs on-site and UCONN Health pricing)
B. Additional Costs (if applicable):
1. CGI Supplied Consumables:
96-well ABI Sequencing Plate: $2.20 (cost for one plate)
Training (CGI, UCONN Storrs on-site and UCONN Health pricing)
A. Workshop Fees:
Flat Rate for Laboratory Workshops (various topics): $850.00
Flat Rate for Bioinformatics Workshops: $500.00
Fluidigm BioMark HD (CGI, UCONN Storrs on-site and UCONN Health pricing)
A. Instrument Access Fee for the Fluidigm BioMark HD:
1. Unassisted/Assisted Instrument Access:
Flat Rate User Access Fee (per array): $155.00
Fluidigm C1 (CGI, UCONN Storrs on-site and UCONN Health pricing)
A. Instrument Access Fee for the Fluidigm C1:
1. Unassisted/Assisted Instrument Access:
Flat Rate User Access Fee (per array): $191.00
B. Single Cell mRNA-Seq:
1. Single Cell Capture and mRNA Library Preparation for Illumina Sequencing:
Please contact the CGI for a price quote
Agilent TapeStation 2200 (CGI, UCONN Storrs on-site and UCONN Health pricing)
A. Instrument Access Fee for the Agilent TapeStation 2200:
1. Unassisted/Assisted Instrument Access:
Flat Rate User Access Fee (per run): $12.00
B. Assay Types (Agilent TapeStation 2200):
1. Genomic DNA
2. High Sensitivity RNA
3. DNA High Sensitivity 1000
4. DNA 5000
Each assay is designed to process a minimum of 2 samples, up to 96. Please contact the CGI for a price based on assay type as well as number of samples.