Center for Genome Innovation – Service Rates (updated January 2025)
- Service rates are based on an internal audit of costs for reagents, consumables and labor and are subject to periodic review. Internal rates for UCONN researchers are shown. Rates billed to external (non-UCONN, not for-profit) adds an additional 35% to all billable services for external users. UCONN adds an additional 100% to all billable services for external for-profit company customers.
- Please contact the CGI for project quotes. Projects can be submitted at any time for any number of samples. Project deliverables will be communicated at the time of sample delivery and are subject to change.
MAIN SERVICES:
Illumina Library Preparation and Sequencing (full-service applications for all Illumina Sequencing platforms). NOTE: Please inquire about costs for your specific project and sample number. Select sample number categories are provided to give an idea of costs.
Library Preparation Services for commonly requested applications (Full Service Rates):
1. Illumina Stranded Total RNA library preparation, includes ribosomal depletion. ——— Number of Samples (top row); Cost per sample (bottom row)
| 1-6 samples | 12 | 24 | 48 |
| $270.28/sample | $206.82/sample | $173.17/sample | $157.31/sample |
2. Illumina Stranded mRNA library preparation, includes polyA selection. ——— Number of Samples (top row); Cost per sample (bottom row)
| 1-6 samples | 12 | 24 | 48 |
| $187.71/sample | $128.87/sample | $98.16/sample | $83.45/sample |
3. TruSeq DNA Nano Library Prep (Covaris Shearing for whole genome shotgun) ——— Number of Samples (top row); Cost per sample (bottom row)
| 1-6 samples | 12 | 24 | 48 |
| $129.55/sample | $87.25/sample | $65.43/sample | $54.85/sample |
4. NexteraXT or NexteraFlex (enzymatic shearing for whole genome shotgun) ——— Number of Samples (top row); Cost per sample (bottom row)
| 1-6 samples | 12 | 24 | 48 |
| $88.42/sample | $73.06/sample | $64.71/sample | $60.87/sample |
5. NexteraFlex for Enrichment (Exome Sequencing) ——— Number of Samples (top row); Cost per sample (bottom row)
| 1-6 samples | 12 | 24 | 48 |
| $239.99/sample | $169.24/sample | $141.10/sample | $127.70/sample |
8. NEBNext Small RNA Library preparation ——— Number of Samples (top row); Cost per sample (bottom row)
| 1-6 samples | 12 | 24 | 48 |
| $206.56/sample | $147.76/sample | $117.06/sample | $102.36/sample |
9. Targeted amplicon/metabarcoding (using custom amplicon primers) ——— Number of Samples (top row); Cost per sample (bottom row)
| 1-24 samples | 48 | 96 |
| $35.57/sample | $31.23/sample | $29.19/sample |
The Center for Genome Innovation also offers NGS sample preparation solutions for the following applications:
- ChIP-Seq (“standard input” and “low input” using Swift Biosciences kits)
- Whole Genome Methylation (using the NEBNext Enzymatic Methyl-Seq kit; EM-Seq)
- Illumina RNA for Enrichment
- Custom Enrichment Panels using KAPA HyperPrep
- Low input RNA-Seq with various TakaraBio kits (mRNA and total RNA options available)
- Genotyping by Sequencing (ddRAD)
- Single Cell RNA-Seq (10x Chromium)
For more information on pricing for these applications or to inquire about assistance in developing an application that is not shown please contact us!
Sequencing Instrument Access Fees for the Illumina NovaSeq 6000:
Sequencing Instrument Access:
Single Sequencing Run Access Fee w/ Labor: $490.00
Sequencing Reagent Kit Costs for the Illumina NovaSeq 6000 (Prices shown are for full runs. Lane sharing is available. Please inquire for single lane costs and other important project considerations):
Reagent costs change yearly and are also subject to a 6.5% charge for shipping. Rates are internal per contract price to UCONN at time of service. Prices below are current through Feb 2025:
NovaSeq SP v1.5 100 cycle = $2,417
NovaSeq SP v1.5 200 cycle = $3,166
NovaSeq SP v1.5 300 cycle = $3,454
NovaSeq SP v1.5 500 cycle = $4,835
NovaSeq S1 v1.5 100 cycle = $4,431
NovaSeq S1 v1.5 200 cycle = $5,538
NovaSeq S1 v1.5 300 cycle = $6,043
NovaSeq S2 v1.5 100 cycle = $8,346
NovaSeq S2 v1.5 200 cycle = $10,362
NovaSeq S2 v1.5 300 cycle = $11,052
NovaSeq S4 v1.5 35 cycle = $11,192
NovaSeq S4 v1.5 200 cycle = $14,879
NovaSeq S4 v1.5 300 cycle = $16,578
Lane Sharing is available on all NovaSeq kits. SP, S1 and S2 flow cells have 2 lanes; S4 flow cells have 4 lanes. An Xp lane loading kit is necessary to separate pools:
Xp 2-lane loading kit = $343.00
Xp 4-lane loading kit = $690.00
Sequencing Instrument Access Fees for the Illumina NextSeq 550:
1. Assisted Sequencing Instrument Access:
Single Sequencing Run Access Fee w/ Labor: $301.00
Sequencing Reagent Kit Costs for the Illumina NextSeq 550:
Reagent costs change yearly and are also subject to a 6.5% charge for shipping. Rates are internal per contract price to UCONN at time of service. Prices below are current through Feb 2024:
Prices available upon request
Sequencing Instrument Access Fees for the Illumina MiSeq:
1. Unassisted Sequencing Instrument Access:
Single Sequencing Run Access Fee: $118.50
2. Assisted Sequencing Instrument Access:
Single Sequencing Run Access Fee w/ Labor: $156.00 (v2/v3 runs); $78.00 (Nano or Micro runs)
Sequencing Reagent Kit Costs for the Illumina MiSeq:
Reagent costs change yearly and are also subject to a 6.5% charge for shipping. Rates are internal per contract price to UCONN at time of service. Prices below are current through Feb 2024:
MiSeq Reagent Kit v2 (50 cycle) = $976.12
MiSeq Reagent Kit v2 (300 cycle) = $1,250.28
MiSeq Reagent Kit v2 (500 cycle) = $1,405.76
MiSeq Reagent Kit v3 (150 cycle) = $1,038.76
MiSeq Reagent Kit v3 (600 cycle) = $1,829.88
MiSeq Reagent Kit Nano v2 (300 cycle) = $347.76
MiSeq Reagent Kit Nano v2 (500 cycle) = $417.68
MiSeq Reagent Kit Micro v2 (300 cycle) = $525.32
Instrument Access Fee for the BioRad CFX 96:
1. Unassisted/Assisted Instrument Access:
Access Fee: $15.00/day
Instrument Access Fee for the 10X Chromium:
1. Unassisted/Assisted Instrument Access:
Access Fee: $100
Please inquire about sample preparation costs for single cell RNA-Seq.
Instrument Access Fee for the BioRad Digital Droplet PCR QX200:
1. Unassisted/Assisted Instrument Access:
Access Fee: $100
Cost for sample processing (EvaGreen or probe-based assays) = $5.00/sample
Sample Extraction Services
Total RNA Extraction (homogenized tissues or cells) = $25.00/sample
RNA Extraction (PAXGene blood tubes) = $33.00/sample
Genomic DNA Extraction = $19.00/sample