In an effort to provide researchers access to the best and most cost effective sequencing services, the Center for Genome Innovation is partnering with Eurofins Genomics for Sanger sequencing solutions.
Eurofins Genomics is an international leader in DNA sequencing and oligonucleotide/gene synthesis, and is known for quick turn around times and excellent quality in both areas. Although each lab has the option to choose from many vendors for Sanger sequencing, Eurofins has given UCONN researchers submitting samples through the CGI competitive pricing for many different sequencing configurations, including convenient services like custom primer synthesis.
Related Information
- For more information on how to prepare samples for submission download the Eurofins Sample Submission Guidelines (pdf)
- For a look at all the services available to you (including oligo and gene synthesis services) download the Eurofins Detailed Pricing Guide for UCONN
- For a detailed explanation of Sanger Services, cost per sample and volume requirements download the Sanger Sequencing from Eurofins Genomics Guide
*** FREQUENTLY ASKED QUESTIONS ***
How do I submit samples?
Each lab or lab member must create an account for sample submission, data delivery and payment via the UCONN Eurofins portal (see below). You will order and submit sequencing reactions (as well as other services) by completing the appropriate information for each sample/primer submitted, receive your data and organize billing through this portal. The CGI does not bill you for these services.
Samples must be prepared according to the sample submission guidelines:
Requested Concentrations for templates and primers:
| Template Type | Template Size | Template [c] ng/uL | Primer [c] pmol/uL |
| Plasmid | < 20Kb | 100 – 200 | 2 – 10 |
| Purified PCR | 100 – 300 bp | 10 – 20 | 2 – 10 |
| Purified PCR | 301 – 1,000 bp | 20 – 40 | 2 – 10 |
| Purified PCR | > 1,000 bp | 40 – 60 | 2 – 10 |
| Large Constructs (BAC) | >20 Kb | 250 – 500 | 10 – 15 |
Requested Volumes for templates and primers – Separate Tube Submissions:
NOTE: See Table above for relevant concentrations
| Single Aliquot | Volume (1st reaction) | Additional Volume (for each additional reaction) |
| Template DNA | 10 uL | 5 uL |
| Primer | 10 uL | 5 uL |
| Power Read DNA | 10 uL | 10 uL |
Requested Volumes for Premix Submissions:
NOTE: See Table above for relevant concentrations
| Template Volume (uL) | Primer Volume (uL) | Total Volume (uL) |
| 8 | 4 | 12 |
All template and primer (separate tubes) and premix material being sent to Eurofins must be in 1.5mL or 2mL microcentrifuge tubes or 96-well full-skirted plates. Tubes or plates must be clearly marked by the customer or affixed with a barcode sticker. Barcode stickers and zip top sample submission bags are available now through the CGI (or can be ordered for free for your lab once you have an account).
Once your samples have been prepared according to the Eurofins submission protocol, simply drop them off in the CGI/Eurofins Dropbox (ESB, outside the 3rd floor elevators) for daily pickup. Sample pickup happens at 3pm.
What are the costs?
| Service | Cost per sample | Description |
| Separate Tube Sequencing | $4.00 | Template and primer sent separately or as premixed samples. Customer supplies consumables. Use of Eurofins standard sequencing primers available. |
| SimpleSeq Tube Sequencing | $4.00 | 96 prepaid tube reactions to submit in batches as needed. Template and primer sent separately or as premixed samples. Barcode tubes provided by Eurofins. Must purchase 96 reactions. Use of Eurofins standard sequencing primers available. |
| SimpleSeq Premix (“Webless”) | $3.50 | 96 prepaid tube reactions to submit in batches as needed. Must be premixed samples. Barcode tubes provided by Eurofins. Must purchase 96 reactions |
| Plate Reaction Submission | $2.00 | Template and primer sent separately or as premixed samples for 24 or more reactions. Customer supplies consumables (plate and caps). Use of Eurofins standard sequencing primers available. |
| PrePaid Plate Reaction | $2.25 | 96 prepaid plate reactions (template can be separate from primer or premixed). Barcode plate and caps provided by Eurofins. Intended for one-time use of all 96 reactions. Use of Eurofins standard sequencing primers available. |
| ShortSeq Plate | $1.96 | Samples sent as premixed with primer for 24 or more reactions. Customer supplies consumables (plate and caps). Expected read lengths of 500 – 700bp. |
| Ready2Load Plate | $0.50 | BigDye Terminated and precipitated samples only in plate format for 24 or more samples. Customer supplies consumables (plate and caps). Expected read lengths of 500 – 700bp. |
How do I place an order for Sanger Sequencing?
- Visit the UCONN custom portal
- Create an account for your lab (you only need to do this once!)
- Before you drop off your samples, submit your order online. Be sure to use the barcode stickers available in the CGI to label your tubes.
- Barcode sticker numbers will be entered into the submission portal along with other information about your samples/primers. If you do not use barcode stickers, you must CLEARLY LABEL your tubes! Unclear markings will delay sample processing.
- Print out order confirmation and enclose with samples in ziptop bag or similar.
- Do not leave your samples in tube racks and be sure to check that all tubes are completely closed!
- Use the sample submission bags provided by Eurofins (available in the CGI).
- Drop off samples in the Eurofins Dropbox (Engineering and Science Building, outside of 3rd floor elevators) by 3:00pm.