NextGen Sequencing Services from Perkin Elmer using the HiSeq 2000
The University of Connecticut has entered into an agreement with Perkin Elmer Corporation to provide Illumina Next Generation sequencing on the HiSeq 2000. Investigators will be responsible for library preparation and data analysis. However, Perkin Elmer (PE) offers library preparation and bioinformatics services at an additional cost. The process for submitting service requests to PE is described below.
- For more information about the services provided through this contract, please visit the UCHC Next Generation Sequencing website.
- For more information about the Illumina Sequencing, please visit our Illumina HiSeq 2500/2000 sequencing page.
- To get an estimate of sequencing coverage for your experiment, download our Sequencing Coverage Calculator.
Sample Submission
UConn/UCHC investigators will be charged for reagent costs for their share of the flow cell, i.e. only the number of lanes utilized for a sequencing run. These services have been negotiated for the UConn/UCHC research communities and do not reflect market pricing and do not extend to UConn or UCHC sub-recipients and external partners.
The steps for sample submission for Storrs Faculty are shown below. To download a pdf version of this document, click here.
Required Materials: Sequencing Request and Submission Process
1. UCONN-Storrs Principal Investigator (PI) submits project details directly to Dawn Casey (dawn.casey@perkinelmer.com). Dawn will work with the PI on project deliverables and will generate a quote.
2. The PI will be given a Project ID from PE as part of the quote.
3. PI/Lab staff downloads and completes the PE Sample Submission Form (Excel Format), located in the Downloadable Documents and Forms section below. Form must include the unique Project ID number (from your quote) as well as the PI’s KFS account the project will be billed to.
4. PI/Lab staff completes the online Internal Submission Form found here, including uploads of both the completed PE Sample Submission Form and the relevant PE project quote.
***Project samples cannot be mailed to PE until the internal form has been approved.***
5. Following approval, samples must be packaged according to PE’s Sample Submission Guidelines (document can be downloaded from the Downloadable Documents and Forms section below). Package must include a hard copy of the PE Sample Submission Form, project quote and any other documents specified in PE’s Sample Submission Guidelines.
6. PI/Lab staff can use their own FedEx mailer and account for shipping. If preferred, the PI/Lab staff can request a pre-printed FedEx mailer (requested through the online Internal Submission form; see step 4).
PI/Lab staff is responsible for scheduling a FedEx pickup, packaging materials, and any applicable insurance, dry ice, tubes, etc.
7. When the samples are ready to ship, the PI/Lab staff will email seqprojects@perkinelmer.com and Bo Reese (bo.reese@uconn.edu), including in the email the PE Sample Submission Form (as attachment) and FedEx tracking number.
8. PE will send notification directly to the PI/Lab Staff upon receipt of the samples.
9. All UCONN-Storrs submissions will undergo incoming sample QC at PE (unless the PI requests otherwise on the PE Sample Submission Form).
10. The CGI will transfer funds from the PI’s KFS account to a holding account for reconciliation with the UCHC Research Finance group after an invoice is received from PE. Once the funds are transferred from the PI’s KFS account, no further action will be required for payment.
Electronic data delivery, storage and analysis
We expect that electronic data delivery will become available in the near future, which will eliminate hard drives and additional shipping charges. Until then, data is typically shipped back to the PI on a hard drive provided by PE (additional cost) or on a hard drive provided by the PI (to be included in the original shipment). The CGI does not offer backup data storage at this time.
If “full-service” bioinformatics support is required, PI’s should contact Dawn Casey for more information about the bioinformatics services PE offers. Alternatively, PI’s can contact staff at the Biotechnology-Bioservices center at UConn, Storrs to inquire about bioinformatics help.
Perkin Elmer Contact
To receive a quote for a new sequencing project, or to get more information about the sequencing services offered through PE contact:
Dawn Casey
Office: +1 781-252-0473
Email: Dawn Casey@perkinelmer.com
Downloadable Documents and Forms
Sequencing Request and Submission Process for Storrs Faculty
Storrs Sample Submission Guidelines August 2014
PE Sample Submission Form for Storrs Faculty (Sample Manifest)
PE Sample Submission Form Revised March 2014
Perkin Elmer Sample Submission and Shipping Guidelines
Perkin Elmer SamplePrep Shipping Guidelines v5-2