University of Connecticut University of UC Title Fallback Connecticut

Instrumentation

CGI_ISG
Center for Genome Innovation Instrumentation

Main Location: 181 Auditorium Road, U3197, Engineering & Science Building, UCONN Storrs Campus
Satellite Location: 400 Farmington Avenue, Farmington, UCONN Health

All instrumentation is accessible to faculty and students pending appropriate training. The extent of training needed before independent use depends on the instrumentation and skill level of the user.  Training is required for all next generation sequencing platforms (if users would like to use instruments independently) and can be met by either attending a workshop or setting up one-on-one training with CGI staff. If individuals are not comfortable working independently, he/she can request supervision from the CGI staff.

In addition to instrument access, the CGI also offers fee-for-service for NextGen sequencing and genotyping services.  A full list of services and associated costs can be found by visiting the CGI Service Rates – FY 2016 – 2017 page. If you have questions or would like more information about any of the services provided by the CGI, contact Bo Reese.

Current molecular biology and genomics instrumentation:

As of 09/20/2017

PCR, Real-Time PCR and Digital PCR:

• ABI GeneAmp 9700 gold block thermocyclers
• BioRad C1000 Thermal Cyclers (deep well reaction modules)
• BioRad CFX96 Real-Time PCR machine
• Fluidigm BioMark HD (for qPCR and Digital PCR)

Sample QC:

• Agilent 2100 Bioanalyzer Automated Electrophoresis system
• Agilent 2200 TapeStation Automated Electrophoresis system
• Thermo-Fisher NanoDrop 2000
• Agarose gel electrophoresis systems
• Turner Biosystems Picofluorometer
• Qubit 3.0

Nucleic Acid Fragmentation/Sample homogenization:

• Digilab Hydroshear
• MP Biomedicals FastPrep 24 Sample Prep System
• Qiagen Tissuelyser II

Array Systems:

• Affymetrix Gene Altas System
• Affymetrix Gene Chip System
• Illumina NextSeq 550 (w/ Infinium Array Scanning capabilities)

Microfluidics (Single Cell Genomics)

• Fluidigm C1 Single-Cell Auto Prep
• Fluidigm Access Array systems (multiple)
• 10X Chromium System (10X Genomics; WGS/Exome and Single Cell Analysis)

Other ancillary equipment:

• IKA UltraTurrax
• Allegra X-12R refrigerated centrifuge
• Benchmark Plate Shaker
• BioRad Gel Doc gel imaging system

DNA Sequencing and Genome Analysis:

• ABI 3500 Genetic Analyzer Capillary Sequencer
• Illumina NextSeq 550 NextGen Sequencer (1)
• Illumina NextSeq 500 NextGen Sequencer (1)
• Illumina MiSeq NextGen Sequencer (2)
• Bionano Irys
• Oxford NanoPore MinION