University of Connecticut University of UC Title Fallback Connecticut

CGI Service Rates – FY 2016 – 2017 (Effective December 01, 2016)

CGI_ISG

Center for Genome Innovation – FEE STRUCTURE Fiscal Year 2016 – 2017

  • These rates are based on an internal audit of costs and are subject to annual review.  Rates for internal UCONN researchers are shown.  Rates billed to external (non-UCONN) customers must include institutional indirect costs even though these costs are not included in the rates for internal University users. The FY16-17 rate adds an additional 58% to billable services.
  • Projects are not limited to the sample numbers shown in each section. For sample pricing greater than numbers shown, please contact the CGI.


MAIN SERVICES:


Illumina Library Preparation and Sequencing (includes applications for the NextSeq 500, MiSeq and HiSeq Sequencing platforms) (CGI, UCONN Storrs on-site and UCONN Health pricing)

A. Library Preparation workflows (Assisted Rates):

1. TruSeq/EpiGnome Methyl-Seq Library Sample Prep including Bisulfite conversion ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)

1 2 3 4 5 6 7 8 9 10 11 12
$493.86 $590.44 $687.01 $783.59 $880.17 $976.74 $1,073.32 $1,169.90 $1,267.45 $1,364.03 $1,460.60 $1,557.18

2. NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible) ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)

1 2 3 4 5 6 7 8 9 10 11 12
$693.58 $781.82 $870.05 $958.29 $1,046.53 $1,134.77 $1,223.00 $1,311.24 $1,401.43 $1,489.66 $1,557.90 $1,666.14

3.  TruSeq Stranded Total RNA Library Prep ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)

1 2 3 4 5 6 7 8 9 10 11 12
$745.74 $881.47 $1,017.19 $1,152.92 $1,288.65 $1,424.37 $1,560.10 $1,695.82 $1,883.50 $1,969.23 $2,104.95 $2,240.68

4. TruSeq DNA Nano Library Prep (low input)——— Number of Samples (top row); Total cost for # of samples shown (bottom row)

1 2 3 4 5 6 7 8 9 10 11 12
$452.53 $498.79 $545.04 $591.30 $637.56 $683.82 $730.07 $776.33 $823.56 $869.82 $916.08 $962.34

5. TruSeq Stranded mRNA Library Sample Prep ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)

1 2 3 4 5 6 7 8 9 10 11 12
$665.49 $735.48 $805.48 $875.47 $945.46 $1,015.46 $1,085.45 $1,155.45 $1,227.39 $1,297.38 $1,367.38 $1,437.37

6. Reduced Representation Bisulfite Sequencing using TruSeq DNA Nano Library Sample Prep ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)

1 2 3 4 5 6 7 8 9 10 11 12
$746.47 $808.25 $870.02 $931.79 $993.57 $1,055.34 $1,117.12 $1,178.89 $1,241.64 $1,303.41 $1,365.19 $1,426.96

7. TruSeq Exome Rapid Capture Library Sample Prep (Human Only) ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)

1 2 3 4 5 6 7 8 9 10 11 12
$641.57 $754.47 $868.07 $981.67 $1,095.26 $1,208.86 $1,322.46 $1,436.06 $1,551.60 $1,665.20 $1,778.80 $1,892.39

8. TruSeq ChIP Library Sample Prep ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)

1 2 3 4 5 6 7 8 9 10 11 12
$515.63 $604.69 $693.75 $782.81 $871.86 $960.92 $1,049.98 $1,139.04 $1,230.04 $1,319.10 $1,408.16 $1,497.22

9. Illumina Amplicon Library Sample Prep (using custom amplicon primers) ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)

1 2 3 4 5 6 7 8 9 10 11 12
$288.12 $314.97 $341.81 $368.65 $395.49 $422.34 $449.18 $476.02 $504.81 $531.65 $558.50 $585.34

B. Sequencing Instrument Access Fees for the Illumina NextSeq 500:

1. Unassisted Sequencing Instrument Access:
Single Sequencing Run Access Fee: $294.00 (Flat Rate)

2. Assisted Sequencing Instrument Access:
Single Sequencing Run Access Fee w/ Labor: $324.00 (Flat Rate)

C. Sequencing Reagent Kit Costs for the Illumina NextSeq 500:

Additional Costs for Full Service Component – choose one kit as applicable for run type: (reagent cost per contract price to UConn at time of service):

Illumina NextSeq 500 – 300 cycle (High Output) – $4,094.00
Illumina NextSeq 500 – 300 cycle (Mid Output) – $1,564.00
Illumina NextSeq 500 – 150 cycle (High Output) – $2,557.60
Illumina NextSeq 500 – 150 cycle (Mid Output) – $975.20
Illumina NextSeq 500 – 75 cycle (High Output) – $1,334.00


D. Sequencing Instrument Access Fees for the Illumina MiSeq:

1. Unassisted Sequencing Instrument Access:
Single Sequencing Run Access Fee: $121.00 (Flat Rate)

2. Assisted Sequencing Instrument Access:
Single Sequencing Run Access Fee w/ Labor: $161.00 (Flat Rate)

E. Sequencing Reagent Kit Costs for the Illumina MiSeq:

Additional Costs for Full Service Component – choose one kit as applicable for run type: (reagent cost per contract price to UConn at time of service):

MiSeq Reagent Kit v2 (50 cycle) – $754.40
MiSeq Reagent Kit v2 (300 cycle) – $961.40
MiSeq Reagent Kit v2 (500 cycle) – $1,076.40
MiSeq Reagent Kit v3 (150 cycle) – $805.00
MiSeq Reagent Kit v3 (600 cycle) – $1,407.60
MiSeq Reagent Kit Nano v2 (300 cycle) – $266.80
MiSeq Reagent Kit Nano v2 (500 cycle) – $322.00
MiSeq Reagent Kit Micro v2 (300 cycle) = $404.80


Affymetrix GeneChip (CGI, UCONN Storrs on-site and UCONN Health pricing)

A. Experiment Options – Assisted Rates (service available through Chromosome Core as per approved CGI rate):

1. Affymetrix GeneChip Cytoscan HD Array ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)

1 2 3 4 5 6 7 8 9 10 11 12
$1,120.19 $1,662.41 $2,204.62 $2,746.84 $3,468.31 $4,042.78 $4,617.25 $5,191.73 $5,858.18 $6,437.55 $7,019.77 $7,601.99

2. Affymetrix GeneChip Whole Transcriptome Human 2.0 ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)

1 2 3 4 5 6 7 8 9 10 11 12
$887.60 $1,256.32 $1,627.54 $2,006.26 $2,403.73 $2,782.44 $3,161.16 $3,539.88 $3,937.35 $4,316.07 $4,694.79 $5,073.51

B. Genotyping Instrument Access Fees for the Affymetrix GeneChip:

1. Unassisted/Assisted Instrument Access Fee:
Single Run Access Fee (Assisted and Unassisted Rate): $238.00 (Flat Rate per experiment)


Affymetrix GeneAltas (CGI, UCONN Storrs on-site and UCONN Health pricing)

A. Experiment Options – Assisted Rates (service available through Chromosome Core as per approved CGI rate):

1.  Affymetrix WT Expression Array ——— Number of Samples (top row); Total cost for # of samples shown (bottom row)

1 2 3 4 5 6 7 8
$1,717.69 $1,856.24 $2,012.76 $2,160.28 $3,087.80 $3,375.32 $3,552.84 $3,670.36

B. Genotyping Instrument Access Fees for the Affymetrix GeneAtlas:

1. Unassisted/Assisted Instrument Access Fee:
Single Run Access Fee (Assisted and Unassisted Rate): $123.00 (Flat Rate per use)


BioRad CFX 96 Real-Time PCR System (CGI, UCONN Storrs on-site and UCONN Health pricing)

A. Instrument Access Fee for the BioRad CFX 96:

1. Unassisted/Assisted Instrument Access:
Single Run Access Fee: $12.00 (Flat Rate per use)

B. Additional Costs (if applicable):

1. CGI Supplied Consumables:
BioRad iQ 96-well Real-time PCR Plate: $5.97 (cost for one plate)
BioRad Optical Sealing Tape: $0.96 (cost for one seal)


Sanger Sequencing and Fragment Analysis (CGI, UCONN Storrs on-site and UCONN Health pricing)

sanger_table

B. Additional Costs (if applicable):

1. CGI Supplied Consumables:
96-well ABI Sequencing Plate: $2.20 (cost for one plate)


Training (CGI, UCONN Storrs on-site and UCONN Health pricing)

A. Workshop Fees:
Flat Rate for Laboratory Workshops (various topics): $850.00
Flat Rate for Bioinformatics Workshops: $500.00


Fluidigm BioMark HD (CGI, UCONN Storrs on-site and UCONN Health pricing)

A. Instrument Access Fee for the Fluidigm BioMark HD:

1. Unassisted/Assisted Instrument Access:
Flat Rate User Access Fee (per array): $155.00


Fluidigm C1 (CGI, UCONN Storrs on-site and UCONN Health pricing)

A. Instrument Access Fee for the Fluidigm C1:

1. Unassisted/Assisted Instrument Access:
Flat Rate User Access Fee (per array): $191.00

B. Single Cell mRNA-Seq:

1. Single Cell Capture and mRNA Library Preparation for Illumina Sequencing:
Please contact the CGI for a price quote


Agilent TapeStation 2200 (CGI, UCONN Storrs on-site and UCONN Health pricing)

A. Instrument Access Fee for the Agilent TapeStation 2200:

1. Unassisted/Assisted Instrument Access:
Flat Rate User Access Fee (per run): $12.00

B. Assay Types (Agilent TapeStation 2200):

1. Genomic DNA
2. High Sensitivity RNA
3. DNA High Sensitivity 1000

Each assay is designed to process a minimum of 2 samples, up to 96. Please contact the CGI for a price based on assay type as well as number of samples.